SrasV1, Main, Exploration, bibRecord, 004790

In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila.

Identifieur interne : 004790 ( Main/Exploration ); précédent : 004789; suivant : 004791

In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila.

Auteurs : S L Alan Wong [République populaire de Chine] ; Yiwei Chen ; Chak Ming Chan ; C S Michael Chan ; Paul K S. Chan ; Y L Chui ; Kwok Pui Fung ; Mary M Y. Waye ; Stephen K W. Tsui ; H Y Edwin Chan

Source :

Biochemical and biophysical research communications [ 0006-291X ] ; 2005.

RBID : pubmed:16212942

Descripteurs français

KwdFr :
- Animal génétiquement modifié, Animaux, Anticorps monoclonaux (immunologie), Clathrine (métabolisme), Cytoplasme (métabolisme), Drosophila (génétique), Endocytose, Humains, Phénotype, Protéines virales (génétique), Protéines virales (métabolisme), Techniques de transfert de gènes, Virus du SRAS (génétique).
MESH :
- génétique : Drosophila, Protéines virales, Virus du SRAS.
- immunologie : Anticorps monoclonaux.
- métabolisme : Clathrine, Cytoplasme, Protéines virales.
- Animal génétiquement modifié, Animaux, Endocytose, Humains, Phénotype, Techniques de transfert de gènes.

English descriptors

KwdEn :
- Animals, Animals, Genetically Modified, Antibodies, Monoclonal (immunology), Clathrin (metabolism), Cytoplasm (metabolism), Drosophila (genetics), Endocytosis, Gene Transfer Techniques, Humans, Phenotype, SARS Virus (genetics), Viral Proteins (genetics), Viral Proteins (metabolism).
MESH :
- chemical , genetics : Viral Proteins.
- chemical , immunology : Antibodies, Monoclonal.
- chemical , metabolism : Clathrin, Viral Proteins.
- genetics : Drosophila, SARS Virus.
- metabolism : Cytoplasm.
- Animals, Animals, Genetically Modified, Endocytosis, Gene Transfer Techniques, Humans, Phenotype.

Abstract

The Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3a locus encodes a 274 a.a. novel protein, and its expression has been confirmed in SARS patients. To study functional roles of 3a, we established a transgenic fly model for the SARS-CoV 3a gene. Misexpression of 3a in Drosophila caused a dominant rough eye phenotype. Using a specific monoclonal antibody, we demonstrated that the 3a protein displayed a punctate cytoplasmic localization in Drosophila as in SARS-CoV-infected cells. We provide genetic evidence to support that 3a is functionally related to clathrin-mediated endocytosis. We further found that 3a misexpression induces apoptosis, which could be modulated by cellular cytochrome c levels and caspase activity. From a forward genetic screen, 78 dominant 3a modifying loci were recovered and the identity of these modifiers revealed that the severity of the 3a-induced rough eye phenotype depends on multiple cellular processes including gene transcriptional regulation.

DOI: 10.1016/j.bbrc.2005.09.098
PubMed: 16212942

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">The Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3a locus encodes a 274 a.a. novel protein, and its expression has been confirmed in SARS patients. To study functional roles of 3a, we established a transgenic fly model for the SARS-CoV 3a gene. Misexpression of 3a in Drosophila caused a dominant rough eye phenotype. Using a specific monoclonal antibody, we demonstrated that the 3a protein displayed a punctate cytoplasmic localization in Drosophila as in SARS-CoV-infected cells. We provide genetic evidence to support that 3a is functionally related to clathrin-mediated endocytosis. We further found that 3a misexpression induces apoptosis, which could be modulated by cellular cytochrome c levels and caspase activity. From a forward genetic screen, 78 dominant 3a modifying loci were recovered and the identity of these modifiers revealed that the severity of the 3a-induced rough eye phenotype depends on multiple cellular processes including gene transcriptional regulation.</div>
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In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila.

In vivo functional characterization of the SARS-Coronavirus 3a protein in Drosophila.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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